Handbook of Fluorescent Dyes and Probes PDF by R. W. Sabnis

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Handbook of Fluorescent Dyes and Probes
by R. W. Sabnis
Handbook of Fluorescent Dyes and Probes

Contents
Preface Xv
About The Author Xvii
Acridine Homodimer 1
Acridine Orange (Ao) 3
Acridine Orange 10-Dodecyl Bromide (Dodecyl-Acridine Orange (Dao)) 10
Acridine Orange 10-Nonyl Bromide (Nonyl-Acridine Orange (Nao)) 12
Alexa Fluor 350 Carboxylic Acid Succinimidyl Ester (Amca-S) 15
Alexa Fluor 430 Carboxylic Acid Succinimidyl Ester 17
Alexa Fluor 488 Carboxylic Acid Succinimidyl Ester 20
Alexa Fluor 514 Carboxylic Acid Succinimidyl Ester 23
Alexa Fluor 532 Carboxylic Acid Succinimidyl Ester 25
Alexa Fluor 555 Carboxylic Acid Succinimidyl Ester 28
Alexa Fluor 594 Carboxylic Acid Succinimidyl Ester 30
Alexa Fluor 633 Carboxylic Acid Succinimidyl Ester 33
Alexa Fluor 660 Carboxylic Acid Succinimidyl Ester 35
Alexa Fluor 680 Carboxylic Acid Succinimidyl Ester 38
Alexa Fluor 700 Carboxylic Acid Succinimidyl Ester 40
Alexa Fluor 750 Carboxylic Acid Succinimidyl Ester 42
Alexa Fluor 790 Carboxylic Acid Succinimidyl Ester 45
9-Amino-6-Chloro-2-Methoxyacridine (Acma) 46
4-Amino-5-Methylamino-2′,7′-Difluorofluorescein (Daffm) 50
4-Amino-5-Methylamino-2′,7′-Difluorofluorescein Diacetate (Daf-Fm Da) 52
7–Amino-4-Methylcoumarin (Amc) (Coumarin 120) (Coumarin 440) 54
8-Aminopyrene-1,3,6-Trisulfonic Acid Trisodium Salt (Apts) 63
7-Anilinocoumarin-4-Acetic Acid (Acaa) 65
9-Anthroylnitrile 66

Bobo 1 69
Bobo 3 71
Bodipy Fl C5-Ceramide (C5-Dmb-Ceramide) 74
Bodipy Fl C5-Ganglioside Gm1 77
Bodipy Fl C5-Lactosylceramide 79
Bodipy Fl C5-Sphingomyelin (C5-Dmb-Sphingomyelin) 81
Bodipy Fl C5-Succinimidyl Ester 83
Bodipy Tr Ceramide 85
Bo-Pro 1 87
Bo-Pro 3 89
4-Bromomethyl-6,7-Dimethoxycoumarin 91
5-(Bromomethyl)Fluorescein 93
4-Bromomethyl-7-Methoxycoumarin 95
Calcein Blue Am 101
5-Carboxy-2′,7′-Dichlorofluorescein 102
6-Carboxy-2′,7′-Dichlorofluorescein 104
5-Carboxy-2′,7′-Dichlorofluorescein Diacetate 106
6-Carboxy-2′,7′-Dichlorofluorescein Diacetate 108
5-Carboxyfluorescein 110
6-Carboxyfluorescein 114
5-Carboxyfluorescein Diacetate 119
6-Carboxyfluorescein Diacetate 121
5-Carboxyfluorescein Succinimidyl Ester 125
6-Carboxyfluorescein Succinimidyl Ester 129
5-Carboxynaphthofluorescein 132
6-Carboxynaphthofluorescein 134
Cascade Blue Acetyl Azide Trisodium Salt 136
Cascade Blue Hydrazide Trisodium Salt 138
3-Cyano-7-Ethoxycoumarin (7-Ethoxycoumarin-3-Carbonitrile) 139
3-Cyano-7-Hydroxycoumarin (3-Cyanoumbelliferone) 141
4′,6-Diamidino-2-Phenylindole Dihydrochloride (Dapi) 145
7-Diethylaminocoumarin-3-Carbonyl Azide 148
7-Diethylaminocoumarin-3-Carboxylic Acid (Dac) (Deac) 149
7-Diethylaminocoumarin-3-Carboxylic Acid Hydrazide (Dcch) 153
7-Diethylaminocoumarin-3-Carboxylic Acid Succinimidyl Ester (Deac Se) 155
4-[4-(Diethylamino)Styryl]-N-Methylpyridinium Iodide (4-Di-2-Asp) 157
3,3′-Diethyloxacarbocyanine Iodide (Dioc2(3)) 160
3,3′-Diheptyloxacarbocyanine Iodide (Dioc7(3)) 163
3,3′-Dihexyloxacarbocyanine Iodide (Dioc6(3)) 165
Dihydrorhodamine 123 (Dhr 123) 169
Dihydrorhodamine 6g (Dhr 6g) 173
6,7-Dimethoxycoumarin-4-Acetic Acid 175
7-Dimethylaminocoumarin-4-Acetic Acid (Dmaca) 176
7-Dimethylaminocoumarin-4-Acetic Acid Succinimidyl Ester (Dmaca Se) 178
7-Dimethylamino-4-Methylcoumarin-3-Isothiocyanate (Dacitc) 180
2-[4-(Dimethylamino)Styryl]-N-Ethylpyridinium Iodide (Daspei) 181
4-[4-(Dimethylamino)Styryl]-N-Methylpyridinium Iodide (4-Di-1-Asp) (Daspmi) 183
3,3′-Dimethyl-𝛂-Naphthoxacarbocyanine Iodide (Jc-9) 186
3,3′-Dipentyloxacarbocyanine Iodide (Dioc5(3)) 187
3,3′-Dipropylthiadicarbocyanine Iodide (Disc3(5)) 189
Ethidium Bromide (Homidium Bromide) 193
Ethidium Homodimer-1 (Ethd-1) 199
Ethidium Homodimer-2 (Ethd-2) 203
Fluorescein 205

Fluorescein Diacetate (Fda) 213
Fluoroscein-5-Isothiocyanate (Fitc) 219
Fluorescein-5-Thiosemicarbazide 224
4-Heptadecyl-7-Hydroxycoumarin (4-Heptadecylumbelliferone) 227
Hexidium Iodide 229
Hoechst 33258 231
Hoechst 33342 238
Hoechst 34580 244
7-Hydroxycoumarin-3-Carboxylic Acid (Umbelliferone-3-Carboxylic Acid) 246
7-Hydroxycoumarin-3-Carboxylic Acid Succinimidyl Ester 250
7-Hydroxy-4-Methylcoumarin-3-Acetic Acid 252
8-Hydroxypyrene-1,3,6-Trisulfonic Acid Trisodium Salt (Hpts) (Pyranine) 253
Jojo 1 263
Jo-Pro 1 265
Lolo 1 267
Lysosensor Blue Dnd 167 269
Lysosensor Blue Dnd 192 271
Lysosensor Green Dnd 153 272
Lysosensor Green Dnd 189 273
Lysosensor Yellow/Blue Dnd 160 (Pdmpo) 275
Marina Blue (6,8-Difluoro-7-Hydroxy-4-Methylcoumarin) (Difmu) 277
7-Methoxycoumarin-3-Carbonyl Azide 280
7-Methoxycoumarin-3-Carboxylic Acid 281
7-Methoxycoumarin-3-Carboxylic Acid Succinimidyl Ester 285
8-Methoxypyrene-1,3,6-Trisulfonic Acid Trisodium Salt (Mpts) 287
Nbd C6-Ceramide 289
Nbd Methylhydrazine (Mnbdh) 293
Nuclear Yellow (Hoechst S 769121) 295
Oregon Green 488 (2′,7′-Difluorofluorescein) 297
Oregon Green 488 Cadaverine 300
Oregon Green 488 Carboxylic Acid 302
Oregon Green 514 Carboxylic Acid 304
Oregon Green 488 Carboxylic Acid Diacetate 306
Oregon Green 488 Carboxylic Acid Succinimidyl Ester 308
Oregon Green 514 Carboxylic Acid Succinimidyl Ester 310
Oregon Green 488 Maleimide 312
Pacific Blue (6,8-Difluoro-7-Hydroxycoumarin-3-Carboxylic Acid) 315
Pacific Blue C5-Maleimide 317
Pacific Blue Succinimidyl Ester 318
Popo 1 320
Popo 3 322
Po-Pro 1 324
Po-Pro 3 326

Propidium Iodide (Pi) 328
1-Pyrenebutanoic Acid Hydrazide (Pbh) 334
1-Pyrenebutanoic Acid Succinimidyl Ester 336
1-Pyrenedecanoic Acid (Pda) 340
1-Pyrenedodecanoic Acid (Pdda) 343
1-Pyrenehexadecanoic Acid 346
1-Pyrenehexanoic Acid (Pha) 348
1-Pyrenesulfonyl Chloride 350
1,3,6,8-Pyrenetetrasulfonic Acid Tetrasodium Salt 352
N-(1-Pyrenyl)Iodoacetamide 355
N-(1-Pyrenyl)Maleimide 357
Qsy 7 Carboxylic Acid Succinimidyl Ester 361
Qsy 9 Carboxylic Acid Succinimidyl Ester 364
Qsy 21 Carboxylic Acid Succinimidyl Ester 366
Redoxsensor Red Cc-1 369
Resazurin Sodium Salt (Alamar Blue) 370
Rhodamine 6g (Rh 6g) 373
Rhodamine 110 (Rh 110) 380
Rhodamine 123 (Rh 123) 384
5,5′,6,6′-Tetrachloro-1,1′,3,3′-Tetraethylbenzimidazolocarbocyanine Iodide (Jc-1) 389
Tetramethylrhodamine Ethyl Ester Perchlorate (Tmre) 394
Tetramethylrhodamine Methyl Ester Perchlorate (Tmrm) 396
To-Pro 1 398
To-Pro 3 400
To-Pro 5 403
Toto 1 405
Toto 3 408
True Blue (True Blue Chloride) 411
Vita Blue 413
Yo-Pro 1 415
Yo-Pro 3 419
Yoyo 1 421
Yoyo 3 425
Appendix A Index Of Cas Registry Numbers 427
Appendix B Index Of Acridines 431
Appendix C Index Of Anthracenes 433
Appendix D Index Of Boron Co-Ordination Compounds/Dyes 435
Appendix E Index Of Coumarins 437
Appendix F Index Of Cyanines/Styryls 439
Appendix G Index Of Heterocycles 441
Appendix H Index Of Pyrenes 443
Appendix I Index Of Xanthenes 445

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